Previous Next


№1' 2021


International Medical Journal, Vol. 27., Iss. 1, 2021, P. 23−26.



Strashok L. A., Khomenko M. A., Osolodchenko T. P.

Kharkiv Medical Academy of Postgraduate Education
Mechnikov Institute of Microbiology and Immunology of the National Academy of Medical Sciences of Ukraine, Kharkiv, Ukraine

Obesity is one of the most common non−infectious diseases worldwide among both adults and children. It is associated with the development of diseases such as metabolic syndrome, type 2 diabetes, non−alcoholic fatty liver disease, cardiovascular disease etc. The mechanisms proposed to explain the development and progression of obesity include chronic low−intensity inflammation, bacterial translocation, and endotoxemia, which may resulted from dysbiosis and increased intestinal permeability. To study anthropometric parameters, levels of zonulin, lipopolysaccharide, interleukin−6 and interleukin−10, indices of the colon microbiota, 74 adolescents with obesity aged 12−17 years were examined. The correlation analysis of anthropometric and laboratory indices, between anthropometric ones and those of microflora of a large intestine depending on sex was performed. It is noted that obesity is accompanied by the formation of intestinal dysbiosis in 78.2 % of patients with a decrease in the obligate microflora and an increase in the conditionally pathogenic microflora. In adolescent patients, a significant rise in interleukin−6 levels and a tendency to increase interleukin−10 levels compared with adolescents with normal weight, which is a sign of low−intensity inflammation. There was a significant increase in zonulin levels in obese adolescents compared with those in the control group, that may be an evidence of increased intestinal permeability. Positive correlations have been reported between the body weight, abdominal fat distribution, and increased intestinal permeability as well as activation of low−intensity inflammation. In obese adolescents, in the presence of dysbiotic disorders, it is advisable to harmonize the diet and style and correct intestinal dysbiosis with the intestinal barrier restoration of.

Key words: zonulin, lipopolysaccharide, interleukins, microbiota, adolescents, obesity.


1. WHO. Commission on ending childhood obesity. Facts and figures on childhood obesity. Geneva, 2017.

2. Wittcopp C., Conroy R. Metabolic syndrome in children and adolescents // Pediatrics in review. 2016. Vol. 37, № 5. P. 193−202. doi:−0095

3. Microbiome and NAFLD: potential influence of aerobic fitness and lifestyle modification / M. Panasevich et al. // Physiol Genomics. 2017. Vol. 49, № 8. P. 385−399. doi:

4. The role of the gut microbiota in NAFLD / C. Leung et al. // Nat. Rev. Gastroenterol. Hepatol. 2016. Vol. 13, № 7. R. 412−425. doi:

5. Fasano A. Zonulin and its regulation of intestinal barrier function: the biological door to inflammation, autoimmunity, and cancer // Physiological reviews. 2011. Vol. 91, № 1. P. 151−175. doi:

6. Ohlsson B., Orho−Melander M., Nilsson P. Higher levels of serum zonulin may rather be associated with increased risk of obesity and hyperlipidemia, than with gastrointestinal symptoms or disease manifestations // Int. J. Mol. Sci. 2017. Vol. 18, № 3. P. 582. doi:

7. Gut microbiota, microinflammation, metabolic profile, and zonulin concentration in obese and normal weight subjects / A. Zak−Gołąb et al. // Int. J. Endocrinol. 2013. Vol. 2013. P. 1−9. doi:

8. The relationship between serum zonulin level and clinical and laboratory parameters of childhood obesity / T. Küme et al. // J. of clinical research in pediatric endocrinology. 2017. Vol. 9, № 1. P. 31−38. doi:

9. Zonulin level, a marker of intestinal permeability, is increased in association with liver enzymes in young adolescents / J. Kim et al. // Clinica Chimica Acta. 2018. Vol. 481. P. 218−224. doi:

10. Relationship between immune parameters and non−alcoholic fatty liver disease in obese children / J. Shi et al. // Indian Pediatrics. 2017. Vol. 54, № 10. P. 825−829. doi:−017−1143−x

11. Elevated tumour necrosis factor−alpha was associated with intima thickening in obese children / L. Bo et al. // Acta Paediatrica. 2017. Vol. 106, № 4. P. 627−633. doi:

12. Interleukin 10 and clustering of metabolic syndrome components in pediatrics / J. Chang et al. // Eur. J. of Clinical Investigation. 2014. Vol. 44, № 4. P. 384−394. doi:

13. Behrooz M., Vaghef−Mehrabany E., Ostadrahimi A. Different spexin level in obese vs normal weight children and its relationship with obesity related risk factors // Nutrition, Metabolism and Cardiovascular Diseases. 2020. Vol. 30, № 4. P. 674−682. doi:

14. Intestinal microbiota and serum LPS level and correlation to fatty liver in obese Egyptian children / N. Ismail et al. // RJPBCS. 2014. Vol. 5, № 6. P. 646−653.

15. Gut microbiota and metabolic endotoxemia in young obese mexican subjects / R. Radilla−Vázquez et al. // Obesity facts. 2016. Vol. 9, № 1. P. 1−11. doi:

16. Endotoxin may not be the major cause of postprandial inflammation in adults who consume a single high−fat or moderately high−fat meal / Z. Mo et al. // The J. of Nutrition. 2020. Vol. 150, № 5. P. 1303−1312. doi:

17. Dietary intervention impact on gut microbial gene richness / A. Cotillard et al. // Nature. 2013. Vol. 500, № 7464. P. 585−588. doi:

18. Childhood obesity and Firmicutes/Bacteroidetes ratio in the gut microbiota: a systematic review / C. Indiani et al. // Child. Obes. 2018. Vol. 14, № 8. P. 501−509. doi:

19. The Firmicutes/Bacteroidetes ratio: a relevant marker of gut dysbiosis in obese patients / F. Magne et al. // Nutrients. 2020. Vol. 12, № 5. P. 1474. doi:

Go on Top