International Medical Journal, Vol. 22., Iss. 3, 2016, P. 24−28.
THE USE OF PLATELET RICH PLASMA FOR COMBINED TREATMENT OF VENOUS TROPHIC ULCERS
Kharkiv Medical Academy of Postgraduate Education, Ukraine
To evaluate the dynamics of venous ulcers healing under influence of platelet rich plasma (PRP), the results of treatment of patients with trophic venous ulcers who were performed hemodynamic correction were analyzed. Biopsies were taken from different parts of the wound surface on days 7 and 28 of treatment. Modern immunohistochemical methods with monoclonal antibodies. According to the received data pathogenetically directed surgical treatment with combined use of PRP accelerate maturation of granulation tissue and accelerate re−epithelialization of the ulcer. The samples demonstrate the appearance of the signs of physiological regeneration (adequate angiogenesis, fibrillogenesis with the correct orientation of collagen fibrils and complete re−epithelialization). This promotes healing venous ulcers and reduces the possibility of relapse. Our findings suggest that the use of surgical treatment with elimination of veno−venous reflux in conjunction with the methods of regenerative medicine in patients with severe varicose veins are pathogenetically justified. Complex treatment with topical application of platelet−rich plasma reduces the healing time of venous trophic ulcers, activates synthesis of the connective tissue without changing its structure, which is manifested by normalization of the ratio of fibrillar collagen types, absence of foci of atypical localization of collagen, adequate angiogenesis.
Key words: platelet rich plasma, venous trophic ulcers, immunohistochemical technique.