International Medical Journal, Vol. 22., Iss. 3, 2016, P. 20−24.
THE INFLUENCE OF WOUND DRESSINGS AND BIOPOLYMER GELS ON THE GROWTH AND VIABILITY OF HUMAN SKIN FIBROBLASTS IN VITRO
Kharkov National Medical University
Kharkiv Medical Academy of Postgraduate Education, Ukraine
To assess cytotoxicity of dressings and biopolymer gels used for wound treatment, the influence of some of them on the growth and viability of human skin fibroblasts (FB) in culture was studied. Human skin FB were innoculated in 12−well culture plates and cultured for 72 hours in CO2 incubator. Seven variants were investigated experimentally: 1 −− FB in the culture medium (control); 2 −− FB + dressing Grassolind (Hartmann); 3 −− FB + washed dressing Grassolind; 4 −− FB + mesh Arma−tura (Ukrtechmed); 5 −− FB + hydrogel dressing "Arma−gel+" (Ukrtechmed); 6 −− FB + fibrin gel; 7 −− FB + collagen gel. Fibrin gel was prepared from human plasma and collagen gel from human collagen (type I), serum, and thrombin. After 72 hours of incubation with the studied samples FB number and viability in each case was evaluated. Adhesive properties of Arma−gel+, fibrin and collagen gels were studied. It was shown that dressing Grassolind neutral (Hartmann) and hydrogel dressing Arma−gel+ (Ukrtechmed) have a strong cytotoxic action on human skin FB in culture. Unlike fibrin and collagen gels, Arma−gel+ does not have adhesive properties even after saturation with culture medium. It was found that propylene mesh Arma−tura (Ukrtechmed) and washed from the ointment mass dressing Grassolind (Hartmann) were non−toxic to cells and in combination with fibrin or collagen gel were promising matrices for FB culture preparation for cytoplasty in wound treatment. Fibrin gel accelerates the growth of skin fibroblasts in culture by 2.5 times compared to the control. Thus, in the complex treatment of wounds and burns, including transplantation of dermal equivalents based on the fibroblasts, cytotoxic properties of some dressings and gels should be considered. Their use simultaneously with cytoplasty is not recommended.
Key words: skin fibroblasts, in vitro culture, dressing, biopolymer gels, cytotoxicity, wounds.