International Medical Journal, Vol. 22., Iss. 2, 2016, P. 93−95.
POLYMERASE CHAIN REACTІON IN DIAGNOSIS OF ACUTE NEUROINFECTIONS IN ADULTS
Kharkiv National Medical University, Ukraine
Research aimed at improving the diagnosis of acute neuroinfections for early correction of complex treatment considering the etiology of the disease can increase its effectiveness and is relevant today. The aim of our study was to evaluate the diagnostic value of PCR in the diagnosis of acute neuroinfections in adults. To achieve this, we analyzed all clinical cases of acute neuroinfections in adults in Kharkiv and Kharkiv region for 2011−2013. To establish the etiology of the disease, in addition to bacteriological studies of CSF and blood, PCR was used to detect enteroviruses, types 1,2 herpes virus, herpes zoster virus, Epstein−Barr virus, type 6 human herpesvirus and cytomegalovirus in CSF of patients with serous neuroinfections. HIV−positive patients also underwent investigation for T. gondii. In purulent lesions, CSF PCR was performed to identify Meningitidis N., S. Pneumonia, H. Influenza, which, according to the literature, are among the most frequent pathogens of neuroinfections in adults. The analysis of the findings allows the conclusion that only using only generally accepted bacteriological and serological methods is insufficient. PCR greatly increases the etiological decoding of acute neuroinfections in HIV−negative and HIV−positive patients. In HIV− patients with CNS symptoms, normal CSF indices do not preclude neuroinfections. In such cases, all possible methods of pathogen identification, including PCR, should be used.
Key words: neuroinfections, polymerase chain reaction, etiological structure.