International Medical Journal, Vol. 21., Iss. 1, 2015, P. 100−104.
MOLECULAR-GENETIC INVESTIGATION OF FACTORS OF GROWTH AT NONSCARRING ALOPECIA
Republican Specialized Scientific and Practical Center of Dermatology and Venereology of Ministry of Health, Tashkent, Republic of Uzbekistan
The work covers the investigation of genetic polymorphism of endothelial growth factor VEGF in patients with nonscarring forms of alopecia for diagnosis and prognosis of the disease, and further development of differential treatment. The purpose of the work was to analyze the distribution of polymorphism of G (−634) C and G (−1154) A of VEGF gene in patients with nonscarring forms of alopecia. The study involved 105 patients with various forms of nonscarring alopecia aged 14−50 years. Of them, alopecia areata was present in 38 (36.2 %), androgenic in 56 (53.3 %), diffuse in 11 (10.5 %). Molecular genetic investigation was conducted at the Laboratory of Medical Genetics, Institute of Hematology and Blood Transfusion of Ministry of Health, Republic of Uzbekistan in 47 patients with alopecia of Uzbek population. For molecular genetic studies G (−634) C and G (−154) A gene of VEGF we developed a test system based on real−time polymerase chain reaction. The control group included 30 apparently healthy donors ethnic Uzbeks living in Tashkent. The results of analysis show that distribution of the incidence of mutant alleles and genotypes of polymorphism of G (−634) C and G (−1154) A gene VEGF in alopecia patients accounted for 8.8 % and 6.0%, respectively. In the control group, the corresponding figures were equal to 1.7 %. Allele A carrying heterozygous type of mutation of VEGF genotype at position G (−1154) A was revealed in 11.8 % of the patients with alopecia. In the controls, the persons carrying mutant allele accounted for 3.3 %. A method of testing polymorphisms of G (−634) C and G (−1154) A VEGF gene based on real−time PCR equal to foreign analogues was developed and tested. The observed distribution of heterozygous genotypes of both loci, reliably meet the expected law of Hardy−Weinberg. Due to the fact that the frequency of heterozygosity of polymorphism G (−634) C and G (−1154) A VEGF gene is not very high, their predictive value is low (AUC = 0.57 and 0.54, respectively) and requires further research in the broader range of the population.
Key words: alopecia, genetics, growth factors.