International Medical Journal, Vol. 20., Iss. 2, 2014, P. 5−8.
ENZYME BLOOD LEVEL DISORDER AS A MARKER OF METABOLIC PROCESSES SEVERITY IN MULTIPLE SCLEROSIS
Kharkiv National Medical University
Dynamics of changes of the membrane−bound enzymes activity in blood of 67 patients with multiple sclerosis depending on the variety and duration of the disease was investigated. Enzymes activity was studied using enzymatic kinetic method on biochemical analyzer Screen aster lab (Hospitex Diagnostics). The analysis of indicators of enzymatic activity in multiple sclerosis in the examined patients showed a significant reduction of creatine phosphokinase level by 56.5%, increased by 38.9% level of alanine aminotransferase vs. the controls, and a tendency to reduction of the levels of aspartate aminotransferase (by 12.5%), alkaline phosphatase (by 10.6%) and increase of gamma−glutamyl transpeptidase level (by 23.9 %). Direction and intensity of enzymatic dysbalance depended on the disease variety and duration. The most expressed changes of enzyme activity were observed in patients with cerebrospinal multiple sclerosis. Increased disease duration was accompanied by progressive reduction of enzyme activity (significant reduction of levels of alkaline phosphatase by 23.2 %, creatine phosphokinase by 73.0 %, alanine aminotransferase by 26.2 % and lactate dehydrogenase by 16.3 %). The revealed enzymatic changes suggested disorders of various intracellular metabolic processes occurring in patients with multiple sclerosis. The most pronounced enzyme activity reduction was observed in patients with cerebrospinal multiple sclerosis and in patients with the disease duration over 10 years, which is a marker of the condition severity, pathological process generalization and depletion of the organism reserve capacity. Decreased level of creatine phosphokinase in patients with all varieties of the disease, regardless of the duration of multiple sclerosis, reflects the degree of reduction of metabolic and energy processes rate in the cells of muscular and nervous tissues, possibly due to increased catabolism and, as a consequence, development of movement disorders.
Key words: multiple sclerosis, enzymes.