International Medical Journal, Vol. 21., Iss. 3, 2015, P. 55−58.
EFFICACY OF CRYOGENICALLY PRESERVED MESENCHYMAL PLACENTA STEM CELLS IN CORRECTION OF OXIDATIVE STATE IN TYPE 2 DIABETES MELLITUS
Kharkiv Medical Academy of Postgraduate education, Ukraine
To assess the degree of oxidative stress in experimental animals, the level of primary products of lipid oxidation, diene conjugates, which characterize early stage of peroxidation was determined. Diene conjugates are toxic metabolites, which have a damaging effect on the lipoproteins, proteins, enzymes and nucleic acids. It was found that introduction of cryogenically preserved mesenchymal placenta stem cells (CPMPSC) reduced oxidative stress, as evidenced by reduction in the concentration of diene conjugates by 34 % (p < 0.001). Inhibition of lipid peroxidation intensity influenced by CPMPSC in this case may be the result of activation of antioxidant protection, as evidenced by the increase of the integral index, almost 2−fold in the total antioxidant activity (p < 0.001). It should be noted that increasing the antiradical activity of the system can result from both its enzymatic activity increase (increase in catalase activity, p < 0.05) and non−enzymatic (increasing the concentration of reduced glutathione, p < 0.05) activity increase. Given the important role of oxidative stress in the pathogenesis of diabetic retinopathy (DR), therapeutic correction of pro/antioxidant balance may be effective in prevention of vision loss associated with DR. The absence of convincing results demonstrating the effectiveness of the use of antioxidants in clinical studies may be due to their systemic mode of administration. In this regard, local application of drugs for the restoration of oxidative status may be a promising direction in the prevention of DR.
Key words: cryogenically preserved mesenchymal placenta stem cells, oxidative stress, diabetic retinopathy, experimental animals.